Enabling QTY Server for Designing Water-Soluble α-Helical Transmembrane Proteins.

ABSTRACT: Membrane proteins, particularly those that are α-helical, such as transporters and G-protein-coupled receptors (GPCRs), have significant biological relevance. However, their expression and purification pose difficulties because of their poor water solubilities, which impedes progress in this field. The QTY method, a code-based protein-engineering approach, was recently developed to produce soluble transmembrane proteins. Here, we describe a comprehensive Web server built for QTY design and its relevance for in silico analyses. Typically, the simple design model is expected to require only 2 to 4 min of computer time, and the library design model requires 2 to 5 h, depending on the target protein size and the number of transmembrane helices. Detailed protocols for using the server wi

SUBMITTER: Tao F 

PROVIDER: S-EPMC8764525 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature