A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds.

ABSTRACT:

Background

A wide range of techniques has been developed over the past decades to characterize amyloid-β (Aβ) pathology in mice. Until now, no method has been established to quantify spatial changes in Aβ plaque deposition due to targeted delivery of substances using ALZET® pumps.

Objective

Development of a methodology to quantify the local distribution of Aβ plaques after intracerebral infusion of compounds.

Methods

We have developed a toolbox to quantify Aβ plaques in relation to intracerebral injection channels using Zeiss AxioVision® and Microsoft Excel® software. For the proof of concept, intracerebral stereotactic surgery was performed in 50-day-old APP-transgenic mice injected with PBS. At the age of 100 days, brains were collected for immunhistological analysi

SUBMITTER: Upite J 

PROVIDER: S-EPMC8764605 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature