MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model.

ABSTRACT:

Background

Airway mucus hypersecretion is one of the important pathological features of chronic obstructive pulmonary disease (COPD). MUC5B is the main mucin expressed in the airways of COPD patients and has been indicated to play an important role in airway defense. However, the specific biological function of MUC5B in COPD and the possible mechanism are not clear.

Methods

We established a COPD model with 24-week-old MUC5B^-/- mice exposed to cigarette smoke and tested our hypothesis through lung function tests, HE and PAS staining, immunohistochemistry (IHC), western blot, q-PCR and ELISA.

Results

Compared with MUC5B^+/+ mice, MUC5B^-/- mice had worse general condition and lung function, increased inflammatory infiltration, reduced gobl

SUBMITTER: Huang X 

PROVIDER: S-EPMC8764756 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature