Direct RNA Nanopore Sequencing of Pseudomonas aeruginosa Clone C Transcriptomes.

ABSTRACT: The transcriptomes of Pseudomonas aeruginosa clone C isolates NN2 and SG17M during the mid-exponential and early stationary phases of planktonic growth were evaluated by direct RNA sequencing on the nanopore platform and compared with established short-read cDNA sequencing on the Illumina platform. Fifty to ninety percent of the sense RNAs turned out to be rRNA molecules, followed by similar proportions of mRNA transcripts and noncoding RNAs. The two platforms detected similar proportions of uncharged tRNAs and 29 yet-undescribed antisense tRNAs. For example, the rarest arginine codon was paired with the most abundant tRNA^Arg, and the tRNA^Arg gene is missing for the most frequent arginine codon. More than 90% of the antisense RNA molecules were complementary to a codi

SUBMITTER: Pust MM 

PROVIDER: S-EPMC8765398 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature