Identification of novel translated small ORFs in Escherichia coli using complementary ribosome profiling approaches.

ABSTRACT: Small proteins of <51 amino acids are abundant across all domains of life but are often overlooked because their small size makes them difficult to predict computationally, and they are refractory to standard proteomic approaches. Ribosome profiling has been used to infer the existence of small proteins by detecting the translation of the corresponding open reading frames (ORFs). Detection of translated short ORFs by ribosome profiling can be improved by treating cells with drugs that stall ribosomes at specific codons. Here, we combine the analysis of ribosome profiling data for Escherichia coli cells treated with antibiotics that stall ribosomes at either start or stop codons. Thus, we identify ribosome-occupied start and stop codons with high sensitivity for ∼400 novel putative O

SUBMITTER: Stringer A 

PROVIDER: S-EPMC8765432 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature