Length-based separation of Bacillus subtilis bacterial populations by viscoelastic microfluidics.

ABSTRACT: In this study, we demonstrated the label-free continuous separation and enrichment of Bacillus subtilis populations based on length using viscoelastic microfluidics. B. subtilis, a gram-positive, rod-shaped bacterium, has been widely used as a model organism and an industrial workhorse. B. subtilis can be arranged in different morphological forms, such as single rods, chains, and clumps, which reflect differences in cell types, phases of growth, genetic variation, and changing environmental factors. The ability to prepare B. subtilis populations with a uniform length is important for basic biological studies and efficient industrial applications. Here, we systematically investigated how flow rate ratio, poly(ethylene oxide) (PEO) concentration, and channel lengt

SUBMITTER: Liu P 

PROVIDER: S-EPMC8766588 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature