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Multiplexed direct detection of barcoded protein reporters on a nanopore array.

ABSTRACT: Detection of specific proteins using nanopores is currently challenging. To address this challenge, we developed a collection of over twenty nanopore-addressable protein tags engineered as reporters (NanoporeTERs, or NTERs). NTERs are constructed with a secretion tag, folded domain and a nanopore-targeting C-terminal tail in which arbitrary peptide barcodes can be encoded. We demonstrate simultaneous detection of up to nine NTERs expressed in bacterial or human cells using MinION nanopore sensor arrays.

SUBMITTER: Cardozo N

PROVIDER: S-EPMC8766897 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Multiplexed direct detection of barcoded protein reporters on a nanopore array.

Cardozo Nicolas N Zhang Karen K Doroschak Kathryn K Nguyen Aerilynn A Siddiqui Zoheb Z Bogard Nicholas N Strauss Karin K Ceze Luis L Nivala Jeff J

Nature biotechnology 20210812 1

Detection of specific proteins using nanopores is currently challenging. To address this challenge, we developed a collection of over twenty nanopore-addressable protein tags engineered as reporters (NanoporeTERs, or NTERs). NTERs are constructed with a secretion tag, folded domain and a nanopore-targeting C-terminal tail in which arbitrary peptide barcodes can be encoded. We demonstrate simultaneous detection of up to nine NTERs expressed in bacterial or human cells using MinION nanopore sensor ...[more]

PMID: 34385692

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