Peritoneal Effluent Cell-Free DNA Sequencing in Peritoneal Dialysis Patients With and Without Peritonitis.

ABSTRACT:

Rationale & objective

Conventional culture can be insensitive for the detection of rare infections and for the detection of common infections in the setting of recent antibiotic usage. Patients receiving peritoneal dialysis (PD) with suspected peritonitis have a significant proportion of negative conventional cultures. This study examines the utility of metagenomic sequencing of peritoneal effluent cell-free DNA (cfDNA) for evaluating the peritoneal effluent in PD patients with and without peritonitis.

Study design

Prospective cohort study.

Setting & participants

We prospectively characterized cfDNA in 68 peritoneal effluent samples obtained from 33 patients receiving PD at a single center from September 2016 to July 2018.

Outcomes

Peritoneal effluent, microbia

SUBMITTER: Burnham P 

PROVIDER: S-EPMC8767090 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature