Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo.

ABSTRACT: Ovothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of the sea urchin Paracentrotus lividus by assessing the localization of the key biosynthetic enzyme OvoA, both at transcript and protein level, and perturbing its protein translation by morpholino antisense oligonucleotide-mediated knockdown experiments. In addition, we explored the possible involvement of ovothiol in the inflammatory response by assessing ovoA gene expression and protein localization following exposure to bacterial lipopolysaccharide. The results of the present stu

SUBMITTER: Milito A 

PROVIDER: S-EPMC8767189 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature