An inducible transposon mutagenesis approach for the intracellular human pathogen Chlamydia trachomatis.

ABSTRACT: Background: Chlamydia trachomatis is a prolific human pathogen that can cause serious long-term conditions if left untreated. Recent developments in Chlamydia genetics have opened the door to conducting targeted and random mutagenesis experiments to identify gene function. In the present study, an inducible transposon mutagenesis approach was developed for C. trachomatis using a self-replicating vector to deliver the transposon-transposase cassette - a significant step towards our ultimate aim of achieving saturation mutagenesis of the Chlamydia genome. Methods: The low transformation efficiency of C. trachomatis necessitated the design of a self-replicating vector carrying the transposon mutagenesis cassette (i.e. the Himar-1 transposon con

SUBMITTER: O'Neill CE 

PROVIDER: S-EPMC8767425 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature