Radiosynthesis of a novel antisense imaging probe targeting LncRNA HOTAIR in malignant glioma.

ABSTRACT:

Background

Long non-coding RNA (LncRNA) HOTAIR was amplified and overexpressed in many human carcinomas, which could serve as a useful target for cancer early detection and treatment. The ^99mTc radiolabeled antisense oligonucleotides (ASON) could visualize the expression of HOTAIR and provide a diagnostic value for malignant tumors. The aim of this study was to evaluate whether liposome-coated antisense oligonucleotide probe ^99mTc-HYNIC-ASON targeting HOTAIR can be used in in vivo imaging of HOTAIR in malignant glioma xenografts.

Methods

The ASON targeting LncRNA HOTAIR as well as mismatched ASON (ASONM) were designed and modified. The radiolabeling of ^99mTc with two probes were via the conjugation of bifunctional chelator HYNIC. Then probes

SUBMITTER: Ren J 

PROVIDER: S-EPMC8767688 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature