Oxidative damage and DNA repair in desiccated recalcitrant embryonic axes of Acer pseudoplatanus L.

ABSTRACT:

Background

Most plants encounter water stress at one or more different stages of their life cycle. The maintenance of genetic stability is the integral component of desiccation tolerance that defines the storage ability and long-term survival of seeds. Embryonic axes of desiccation-sensitive recalcitrant seeds of Acer pseudoplatnus L. were used to investigate the genotoxic effect of desiccation. Alkaline single-cell gel electrophoresis (comet assay) methodology was optimized and used to provide unique insights into the onset and repair of DNA strand breaks and 8-oxo-7,8-dihydroguanine (8-oxoG) formation during progressive steps of desiccation and rehydration.

Results

The loss of DNA integrity and impairment of damage repair were significant predictors of the viability of emb

SUBMITTER: Plitta-Michalak BP 

PROVIDER: S-EPMC8767751 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature