Development of a Multiplex PCR Short Tandem Repeat Typing Scheme for Candida krusei.

ABSTRACT: Candida krusei is a human-pathogenic yeast that can cause candidemia with the lowest 90-day survival rate in comparison to other Candida species. Infections occur frequently in immunocompromised patients, and several C. krusei outbreaks in health care facilities have been described. Here, we developed a short tandem repeat (STR) typing scheme for C. krusei to allow the fast and cost-effective genotyping of an outbreak and compared the identified relatedness of 10 isolates to single nucleotide polymorphism (SNP) calling from whole-genome sequencing (WGS). From a selection of 14 novel STR markers, 6 were used to develop two multiplex PCRs. Additionally, three previously reported markers were selected for a third multiplex PCR. In total, 119 C. krusei isolates were typed using these ni

SUBMITTER: van Haren MHI 

PROVIDER: S-EPMC8769724 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature