Subcellular RNA-seq for the Analysis of the Dendritic and Somatic Transcriptomes of Single Neurons.

ABSTRACT: In neurons, local translation in dendritic and axonal compartments allows for the fast and on-demand modification of the local proteome. As the last few years have witnessed dramatic advancements in our appreciation of the brain's neuronal diversity, it is increasingly relevant to understand how local translation is regulated according to cell type. To this end, both sequencing-based and imaging-based techniques have recently been reported. Here, we present a subcellular single cell RNA sequencing protocol that allows molecular quantification from the soma and dendrites of single neurons, and which can be scaled up for the characterization of several hundreds to thousands of neurons. Somata and dendrites of cultured neurons are dissected using laser capture microdissection, followed by cel

SUBMITTER: Perez JD 

PROVIDER: S-EPMC8769762 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature