Toll-like receptor 4-mediated necroptosis in the development of necrotizing enterocolitis.

ABSTRACT:

Background

Dramatic intestinal epithelial cell death leading to barrier dysfunction is one of the mechanism of neonatal necrotizing enterocolitis (NEC), in which Toll-like receptor 4 (TLR4) plays a pivotal role. This study explored the role of necroptosis, a drastic way of cell death in NEC.

Methods

The expression of necroptotic proteins was tested in NEC intestinal tissue and compared with controls. NEC was induced in neonatal wild-type mice and a necroptosis inhibitor was given to investigate whether NEC could be relieved. The general condition, macroscopic scoring, and histological evaluations were performed. The expression of tight junction proteins, inflammatory cytokines, and necroptosis-related proteins was measured, and barrier function was examined. Then, NEC was in

SUBMITTER: Liu T 

PROVIDER: S-EPMC8770135 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature