Order-Unity ¹³ C Nuclear Polarization of [1-¹³ C]Pyruvate in Seconds and the Interplay of Water and SABRE Enhancement.

ABSTRACT: Signal Amplification By Reversible Exchange in SHield Enabled Alignment Transfer (SABRE-SHEATH) is investigated to achieve rapid hyperpolarization of ¹³ C₁ spins of [1-¹³ C]pyruvate, using parahydrogen as the source of nuclear spin order. Pyruvate exchange with an iridium polarization transfer complex can be modulated via a sensitive interplay between temperature and co-ligation of DMSO and H₂ O. Order-unity ¹³ C (>50 %) polarization of catalyst-bound [1-¹³ C]pyruvate is achieved in less than 30 s by restricting the chemical exchange of [1-¹³ C]pyruvate at lower temperatures. On the catalyst bound pyruvate, 39 % polarization is measured using a 1.4 T NMR spectrometer, and extrapolated to >50 % at the end of build-

SUBMITTER: Adelabu I 

PROVIDER: S-EPMC8770613 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature