MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma.

ABSTRACT: MicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method. After 7 days of differentiation culture, whole-cell currents were recorded. The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance, increased potassium current density and decreased calcium current density. Knockdown of miR-142a-3p reduced sodium ion channel current density. The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells, suggesting its possible roles in musc

SUBMITTER: Gu XY 

PROVIDER: S-EPMC8771090 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature