A Model for Allosteric Communication in Drug Transport by the AcrAB-TolC Tripartite Efflux Pump.

ABSTRACT: RND family efflux pumps are complex macromolecular machines involved in multidrug resistance by extruding antibiotics from the cell. While structural studies and molecular dynamics simulations have provided insights into the architecture and conformational states of the pumps, the path followed by conformational changes from the inner membrane protein (IMP) to the periplasmic membrane fusion protein (MFP) and to the outer membrane protein (OMP) in tripartite efflux assemblies is not fully understood. Here, we investigated AcrAB-TolC efflux pump's allostery by comparing resting and transport states using difference distance matrices supplemented with evolutionary couplings data and buried surface area measurements. Our analysis indicated that substrate binding by the IMP triggers quaternary

SUBMITTER: Webber A 

PROVIDER: S-EPMC8773123 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature