Large-Scale Functional Genomics Screen to Identify Modulators of Human β-Cell Insulin Secretion.

ABSTRACT: Type 2 diabetes (T2D) is a chronic metabolic disorder affecting almost half a billion people worldwide. Impaired function of pancreatic β-cells is both a hallmark of T2D and an underlying factor in the pathophysiology of the disease. Understanding the cellular mechanisms regulating appropriate insulin secretion has been of long-standing interest in the scientific and clinical communities. To identify novel genes regulating insulin secretion we developed a robust arrayed siRNA screen measuring basal, glucose-stimulated, and augmented insulin secretion by EndoC-βH1 cells, a human β-cell line, in a 384-well plate format. We screened 521 candidate genes selected by text mining for relevance to T2D biology and identified 23 positive and 68 negative regulators of insulin secretion. Among these,

SUBMITTER: Szczerbinska I 

PROVIDER: S-EPMC8773179 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature