TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.

ABSTRACT: Human telomerase is a specialized DNA polymerase whose catalytic core includes both TERT and human telomerase RNA (hTR). Telomerase in humans, which is silent in most somatic cells, is activated to maintain the telomere length (TEL) in various types of cancer cells, including melanoma. In the vast majority of tumor cells, the TERT promoter is mutated to promote proliferation and inhibit apoptosis. Here, we exploited NG-ABEmax to revert TERT -146 T to -146 C in melanoma, and successfully obtained TERT promoter revertant mutant cells. These TERT revertant mutant cells exhibited significant growth inhibition both in vitro and in vivo. Moreover, A375^-146C/C cells exhibited telomere shortening and the downregulation of TERT at both the transcription and protein levels, and migration

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8773187 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature