Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.

ABSTRACT: In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

SUBMITTER: Bellin T 

PROVIDER: S-EPMC87735 | biostudies-literature | 2001 Jan

REPOSITORIES: biostudies-literature