Functional and Kinetic Comparison of Alanine Cysteine Serine Transporters ASCT1 and ASCT2.

ABSTRACT: Neutral amino acid transporters ASCT1 and ASCT2 are two SLC1 (solute carrier 1) family subtypes, which are specific for neutral amino acids. The other members of the SLC1 family are acidic amino acid transporters (EAATs 1-5). While the functional similarities and differences between the EAATs have been well studied, less is known about how the subtypes ASCT1 and 2 differ in kinetics and function. Here, by performing comprehensive electrophysiological analysis, we identified similarities and differences between these subtypes, as well as novel functional properties, such as apparent substrate affinities of the inward-facing conformation (in the range of 70 μM for L-serine as the substrate). Key findings were: ASCT1 has a higher apparent affinity for Na⁺, as well as a larger [Na

SUBMITTER: Wang J 

PROVIDER: S-EPMC8773628 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature