Generation and Application of Inducible Chimeric RNA ASTN2-PAPPA_as Knockin Mouse Model.

ABSTRACT: Chimeric RNAs (chiRNAs) play many previously unrecognized roles in different diseases including cancer. They can not only be used as biomarkers for diagnosis and prognosis of various diseases but also serve as potential therapeutic targets. In order to better understand the roles of chiRNAs in pathogenesis, we inserted human sequences into mouse genome and established a knockin mouse model of the tamoxifen-inducible expression of ASTN2-PAPPA antisense chimeric RNA (A-P_aschiRNA). Mice carrying the A-P_aschiRNA knockin gene do not display any apparent abnormalities in growth, fertility, histological, hematopoietic, and biochemical indices. Using this model, we dissected the role of A-P_aschiRNA in chemical carcinogen 4-nitroquinoline

SUBMITTER: Luo Y 

PROVIDER: S-EPMC8773765 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature