Molecular Determinants of Selectivity in Disordered Complexes May Shed Light on Specificity in Protein Condensates.

ABSTRACT: Biomolecular condensates challenge the classical concepts of molecular recognition. The variable composition and heterogeneous conformations of liquid-like protein droplets are bottlenecks for high-resolution structural studies. To obtain atomistic insights into the organization of these assemblies, here we have characterized the conformational ensembles of specific disordered complexes, including those of droplet-driving proteins. First, we found that these specific complexes exhibit a high degree of conformational heterogeneity. Second, we found that residues forming contacts at the interface also sample many conformations. Third, we found that different patterns of contacting residues form the specific interface. In addition, we observed a wide range of sequence motifs mediating disorde

SUBMITTER: Monzon AM 

PROVIDER: S-EPMC8773858 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature