Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology.

ABSTRACT: There is a rising interest in biotechnology for the compartmentalization of biochemical reactions in water droplets. Several applications, such as the widely used digital PCR, seek to encapsulate a single molecule in a droplet to be amplified. Directed evolution, another technology with growing popularity, seeks to replicate what happens in nature by encapsulating a single gene and the protein encoded by this gene, linking genotype with phenotype. Compartmentalizing reactions in droplets also allows the experimentalist to run millions of different reactions in parallel. Compartmentalization requires a fluid that is immiscible with water and a surfactant to stabilize the droplets. While there are fluids and surfactants on the market that have been used to accomplish encapsulation, there are

SUBMITTER: Laos R 

PROVIDER: S-EPMC8775225 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature