Tk-deleted Pseudorabies Virus Retains High Pathogenicity in Rats.

ABSTRACT:

Introduction

The pseudorabies virus (PRV) gene encoding thymidine kinase (tk) is an important virulence-associated factor. Attenuation of PRV in susceptible animals is a frequent result of tk deletion. The aim of the study was to assess the pathogenicity of tk-deleted PRV in rats.

Material and methods

Sprague Dawley rats were infected with the tk-deleted PRV strain SuHV-1 ΔTK:247via intranasal or intramuscular inoculation. PRV loads in ten tissues from dead and euthanised rats were determined using real-time PCR.

Results

Infection with SuHV-1 ΔTK:247 could cause death in rats. The 50% lethal dose (LD₅₀) of SuHV-1 ΔTK:247 via intranasal inoculation was 10^3.16 TCID₅₀ in rats. Intramuscular inoculation required a higher dose

SUBMITTER: Zhang L 

PROVIDER: S-EPMC8775734 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature