Multiphoton Bleaching of Red Fluorescent Proteins and the Ways to Reduce It.

ABSTRACT: Red fluorescent proteins and biosensors built upon them are potentially beneficial for two-photon laser microscopy (TPLM) because they can image deeper layers of tissue, compared to green fluorescent proteins. However, some publications report on their very fast photobleaching, especially upon excitation at 750-800 nm. Here we study the multiphoton bleaching properties of mCherry, mPlum, tdTomato, and jREX-GECO1, measuring power dependences of photobleaching rates K at different excitation wavelengths across the whole two-photon absorption spectrum. Although all these proteins contain the chromophore with the same chemical structure, the mechanisms of their multiphoton bleaching are different. The number of photons required to initiate a photochemical reaction varies, depending on waveleng

SUBMITTER: Drobizhev M 

PROVIDER: S-EPMC8775990 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature