In vivo hyperCEST imaging: Experimental considerations for a reliable contrast.

ABSTRACT:

Purpose

HyperCEST contrast relies on the reduction of the solvent signal after selective saturation of the solute magnetization. The scope of this work is to outline the experimental conditions needed to obtain a reliable hyperCEST contrast in vivo, where the "solvent" signal (ie, the dissolved-phase signal) may change over time due to the increase in xenon (Xe) accumulation into tissue.

Methods

Hyperpolarized ¹²⁹ Xe was delivered to mice at a constant volume and rate using a mechanical ventilator, which triggered the saturation, excitation, and acquisition of the MR signal during the exhale phase of the breath cycle-either every breath or every 2, 3, or 4 breaths. Serial Z-spectra and hyperCEST images were acquired before and after a bolus injection of cucurbit[6

SUBMITTER: McHugh CT 

PROVIDER: S-EPMC8776610 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature