Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors.

ABSTRACT: Industrial fermentation processes strive for high robustness to ensure optimal and consistent performance. Medium components, fermentation products, and physical perturbations may cause stress and lower performance. Cellular stress elicits a range of responses, whose extracellular manifestations have been extensively studied; whereas intracellular aspects remain poorly known due to lack of tools for real-time monitoring. Genetically encoded biosensors have emerged as promising tools and have been used to improve microbial productivity and tolerance toward industrially relevant stresses. Here, fluorescent biosensors able to sense the yeast intracellular environment (pH, ATP levels, oxidative stress, glycolytic flux, and ribosome production) were implemented into a versatile and easy-to-use

SUBMITTER: Torello Pianale L 

PROVIDER: S-EPMC8776715 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature