Development of a Phage Cocktail to Target Salmonella Strains Associated with Swine.

ABSTRACT: Infections caused by multidrug resistant Salmonella strains are problematic in swine and are entering human food chains. Bacteriophages (phages) could be used to complement or replace antibiotics to reduce infection within swine. Here, we extensively characterised six broad host range lytic Salmonella phages, with the aim of developing a phage cocktail to prevent or treat infection. Intriguingly, the phages tested differed by one to five single nucleotide polymorphisms. However, there were clear phenotypic differences between them, especially in their heat and pH sensitivity. In vitro killing assays were conducted to determine the efficacy of phages alone and when combined, and three cocktails reduced bacterial numbers by ~2 × 10³ CFU/mL within two hours. Th

SUBMITTER: Thanki AM 

PROVIDER: S-EPMC8777603 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature