Evaluation of an ASFV RNA Helicase Gene A859L for Virus Replication and Swine Virulence.

ABSTRACT: African swine fever virus (ASFV) is producing a devastating pandemic that, since 2007, has spread to a contiguous geographical area from central Europe to Asia. In July 2021, ASFV was detected in the Dominican Republic, the first report of the disease in the Americas in more than 40 years. ASFV is a large, highly complex virus harboring a large dsDNA genome that encodes for more than 150 genes. The majority of these genes have not been functionally characterized. Bioinformatics analysis predicts that ASFV gene A859L encodes for an RNA helicase, although its function has not yet been experimentally assessed. Here, we evaluated the role of the A859L gene during virus replication in cell cultures and during infection in swine. For that purpose, a recombinant virus (ASFV-G-∆A859L) harboring a

SUBMITTER: Ramirez-Medina E 

PROVIDER: S-EPMC8777736 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature