Genetic Engineering of Streptomyces ghanaensis ATCC14672 for Improved Production of Moenomycins.

ABSTRACT: Streptomycetes are soil-dwelling multicellular microorganisms famous for their unprecedented ability to synthesize numerous bioactive natural products (NPs). In addition to their rich arsenal of secondary metabolites, Streptomyces are characterized by complex morphological differentiation. Mostly, industrial production of NPs is done by submerged fermentation, where streptomycetes grow as a vegetative mycelium forming pellets. Often, suboptimal growth peculiarities are the major bottleneck for industrial exploitation. In this work, we employed genetic engineering approaches to improve the production of moenomycins (Mm) in Streptomyces ghanaensis, the only known natural direct inhibitors of bacterial peptidoglycan glycosyltransferses. We showed that in vivo elimination of binding sites for

SUBMITTER: Makitrynskyy R 

PROVIDER: S-EPMC8778134 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature