Direct RNA Nanopore Sequencing of SARS-CoV-2 Extracted from Critical Material from Swabs.

ABSTRACT: In consideration of the increasing prevalence of COVID-19 cases in several countries and the resulting demand for unbiased sequencing approaches, we performed a direct RNA sequencing (direct RNA seq.) experiment using critical oropharyngeal swab samples collected from Italian patients infected with SARS-CoV-2 from the Palermo region in Sicily. Here, we identified the sequences SARS-CoV-2 directly in RNA extracted from critical samples using the Oxford Nanopore MinION technology without prior cDNA retrotranscription. Using an appropriate bioinformatics pipeline, we could identify mutations in the nucleocapsid (N) gene, which have been reported previously in studies conducted in other countries. In conclusion, to the best of our knowledge, the technique used in this study has not been used f

SUBMITTER: Vacca D 

PROVIDER: S-EPMC8778588 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature