High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence.

ABSTRACT: A broad array of imaging and diagnostic technologies employs fluorophore-labeled antibodies for biomarker visualization, an experimental technique known as immunofluorescence. Significant performance advantages, such as higher signal-to-noise ratio, are gained if the appended fluorophore emits near-infrared (NIR) light with a wavelength >700 nm. However, the currently available NIR fluorophore antibody conjugates are known to exhibit significant limitations, including low chemical stability and photostability, weakened target specificity, and low fluorescence brightness. These fluorophore limitations are resolved by employing a NIR heptamethine cyanine dye named s775z whose chemical structure is very stable, charge-balanced, and sterically shielded. Using indirect immunofluorescence for im

SUBMITTER: Schreiber CL 

PROVIDER: S-EPMC8779000 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature