MDrop-Seq: Massively Parallel Single-Cell RNA-Seq of <i>Saccharomyces cerevisiae</i> and <i>Candida albicans</i>.

ABSTRACT: Advances in high-throughput single-cell RNA sequencing (scRNA-seq) have been limited by technical challenges such as tough cell walls and low RNA quantity that prevent transcriptomic profiling of microbial species at throughput. We present microbial Drop-seq or mDrop-seq, a high-throughput scRNA-seq technique that is demonstrated on two yeast species, Saccharomyces cerevisiae, a popular model organism, and Candida albicans, a common opportunistic pathogen. We benchmarked mDrop-seq for sensitivity and specificity and used it to profile 35,109 S. cerevisiae cells to detect variation in mRNA levels between them. As a proof of concept, we quantified expression differences in heat shock S. cerevisiae using mDrop-seq. We detected differential activation of stress resp

SUBMITTER: Dohn R 

PROVIDER: S-EPMC8779198 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature