Exploring the Tunability and Dynamic Properties of MarR-PmarO Sensor System in Escherichia coli.

ABSTRACT: Transcriptional factor-based biosensors (TFBs) have been widely used in dynamic pathway control or high-throughput screening. Here, we systematically explored the tunability of a salicylic acid responsive regulator MarR from Escherichia coli aiming to explore its engineering potential. The effect of endogenous MarR in E. coli on the MarR-PmarO biosensor system was investigated. Furthermore, to investigate the function of marO binding boxes in this biosensor system, a series of hybrid promoters were constructed by placing the marO binding boxes in the strong constitutive pL promoter. The engineered hybrid promoters became responsive to MarR and salicylic acid. To further study the influence of each nucleotide in the marO box on MarR binding, we employed dynamic modeling to sim

SUBMITTER: Zou Y 

PROVIDER: S-EPMC8779632 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature