Transplantation of patient-specific bile duct bioengineered with chemically reprogrammed and microtopographically differentiated cells.

ABSTRACT: Cholangiopathy is a diverse spectrum of chronic progressive bile duct disorders with limited treatment options and dismal outcomes. Scaffold- and stem cell-based tissue engineering technologies hold great promise for reconstructive surgery and tissue repair. Here, we report a combined application of 3D scaffold fabrication and reprogramming of patient-specific human hepatocytes to produce implantable artificial tissues that imitate the mechanical and biological properties of native bile ducts. The human chemically derived hepatic progenitor cells (hCdHs) were generated using two small molecules A83-01 and CHIR99021 and seeded inside the tubular scaffold engineered as a synergistic combination of two layers. The inner electrospun fibrous layer was made of nanoscale-macroscale polycaprolacto

SUBMITTER: Buisson EM 

PROVIDER: S-EPMC8780056 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature