A Novel Real Time PCR Method for the Detection and Quantification of <i>Didymella pinodella</i> in Symptomatic and Asymptomatic Plant Hosts.

ABSTRACT: Didymella pinodella is the major pathogen of the pea root rot complex in Europe. This wide host range pathogen often asymptomatically colonizes its hosts, making the control strategies challenging. We developed a real-time PCR assay for the detection and quantification of D. pinodella based on the TEF-1 alpha gene sequence alignments. The assay was tested for specificity on a 54-isolate panel representing 35 fungal species and further validated in symptomatic and asymptomatic pea and wheat roots from greenhouse tests. The assay was highly consistent across separate qPCR reactions and had a quantification/detection limit of 3.1 pg of target DNA per reaction in plant tissue. Cross-reactions were observed with DNA extracts of five Didymella species. The risk of cro

SUBMITTER: Sisic A 

PROVIDER: S-EPMC8780382 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature