M⁶ A reader protein YTHDF2 regulates spermatogenesis by timely clearance of phase-specific transcripts.

ABSTRACT:

Objectives

Accumulating evidences show that the regulatory network of m⁶ A modification is essential for mammalian spermatogenesis. However, as an m⁶ A reader, the roles of YTHDF2 remain enigmatic due to the lack of a proper model. Here, we employed the germ cell conditional knockout mouse model and explored the function of YTHDF2 in spermatogenesis.

Materials and methods

Ythdf2 germ cell conditional knockout mice were obtained by crossing Ythdf2-floxed mice with Vasa-Cre and Stra8-Cre mice. Haematoxylin and eosin (HE) staining, immunofluorescent staining and Western blotting were used for phenotyping. CASA, IVF and ICSI were applied for sperm function analysis. RNA-seq, YTHDF2-RIP-seq and quantitative real-time PCR were used to explore transcriptome ch

SUBMITTER: Qi M 

PROVIDER: S-EPMC8780898 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature