ClC-c regulates the proliferation of intestinal stem cells via the EGFR signalling pathway in Drosophila.

ABSTRACT:

Objectives

Adult stem cells uphold a delicate balance between quiescent and active states, which is crucial for tissue homeostasis. Whereas many signalling pathways that regulate epithelial stem cells have been reported, many regulators remain unidentified.

Materials and methods

Flies were used to generate tissue-specific gene knockdown and gene knockout. qRT-PCR was used to assess the relative mRNA levels. Immunofluorescence was used to determine protein localization and expression patterns. Clonal analyses were used to observe the phenotype. RNA-seq was used to screen downstream mechanisms.

Results

Here, we report a member of the chloride channel family, ClC-c, which is specifically expressed in Drosophila intestinal stem/progenitor cells and regulates intestinal st

SUBMITTER: Huang J 

PROVIDER: S-EPMC8780901 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature