YTHDF1 promotes mRNA degradation via YTHDF1-AGO2 interaction and phase separation.

ABSTRACT:

Objectives

YTHDF1 is known as a m⁶ A reader protein, and many researches of YTHDF1 focused on the regulation of mRNA translation efficiency. However, YTHDF1 is also related to RNA degradation, but how YTHDF1 regulates mRNA degradation is indefinite. Liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in mammal cells, and there are few reports focused on the correlation of RNA degradation with LLPS. In this research, we focused on the mechanism of YTHDF1 degraded mRNA through LLPS.

Materials and methods

The CRISPR/Cas9 knock out system was used to establish the YTHDF1 knock out (YTHDF1-KO) cell lines (HEK293 and HeLa) and METTL14 knock out (METTL14-KO) cell line (HEK293). 4SU-TT-seq was used to check the half-life changes of m

SUBMITTER: Li J 

PROVIDER: S-EPMC8780909 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature