Comprehensive analysis of human chorionic membrane extracts regulating mesenchymal stem cells during osteogenesis.

ABSTRACT:

Objective

Human chorionic membrane extracts (CMEs) from placenta are known to be a natural biomaterial for bone regeneration, with their excellent osteogenic efficacy on osteoblasts. However, little is known about the regulatory mechanism involved.

Methods and results

We have shown the in vitro and in vivo bone-forming ability of CME using human osteoblasts and bone defect animal models, suggesting that CME greatly enhances osteogenesis by providing an osteoconductive environment for the osteogenesis of osteoblasts. Proteomic analysis revealed that CME contained several osteogenesis-related stimulators such as osteopontin, osteomodulin, Thy-1, netrin 4, retinol-binding protein and DJ-1. Additionally, 23 growth factors/growth factor-related proteins were found in CME, which m

SUBMITTER: Go YY 

PROVIDER: S-EPMC8780910 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature