Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.

ABSTRACT:

Objectives

Implantation failure is a major cause of prenatal mortality. The uterine lumen closure contributes to embryo adhesion to the uterus, but its underlying mechanisms are largely unknown. Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs. The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.

Materials and methods

Uterine and endometrium tissues during implantation were collected in pigs. MALDI-TOF MS was used to characterize the N-glycomic profiles. Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healin

SUBMITTER: Han K 

PROVIDER: S-EPMC8780930 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature