Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer.

ABSTRACT:

Background

Radiotherapy is a highly effective treatment for cervical cancer. Recent studies focused on the radiotherapy induced anti-tumor immunity. Whether tumor-derived extracellular vesicles (EVs) play roles in radiotherapy induced tumor associated macrophage (TAM) polarization remains unclear.

Materials and methods

This study analysed the phenotype of macrophages in cancer tissue and peripheral blood of cervical cancer patients using flow cytometry analysis. The role of EVs from plasma of post-irradiated patients on M2-like transformed macrophages was assessed. The M1- and M2-like macrophages were assessed by expression of cell surface markers (CCR7, CD163) and intracellular cytokines (IL-10, TNFα and iNOS). The capacity of phagocytosis was assessed by PD-1 expression an

SUBMITTER: Ren J 

PROVIDER: S-EPMC8781113 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature