Spatially Mapping the Baseline and Bisphenol-A Exposed <i>Daphnia magna</i> Lipidome Using Desorption Electrospray Ionization-Mass Spectrometry.

ABSTRACT: Untargeted lipidomics has previously been applied to the study of daphnids and the discovery of biomarkers that are indicative of toxicity. Typically, liquid chromatography-mass spectrometry is used to measure the changes in lipid abundance in whole-body homogenates of daphnids, each only ca. 3 mm in length which limits any biochemical interpretation of site-specific toxicity. Here, we applied mass spectrometry imaging of Daphnia magna to combine untargeted lipidomics with spatial resolution to map the molecular perturbations to defined anatomical regions. A desorption electrospray ionization-mass spectrometry (DESI-MS) method was optimized and applied to tissue sections of daphnids exposed to bisphenol-A (BPA) compared to unexposed controls, generating an untargeted mass spectrum a

SUBMITTER: Smith MJ 

PROVIDER: S-EPMC8781255 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature