Genome survey sequencing of the phyto-parasitic nematode Hoplolaimus galeatus.

ABSTRACT:

Background

Hoplolaimus galeatus is a plant-parasite nematode with a broad range of hosts. This nematode is known to damage cotton, corn, and soybean crops. Hoplolaimus galeatus is also an economically important pest of turfgrasses. Despite its economical importance, no genomic resources exist for this parasite.

Methods

Using 300 bp paired-end short read sequencing, this study estimated genome size, analyzed a nearly complete mitochondrial chromosome, and explored nuclear repetitive elements, including microsatellites, in H. galeatus for the first time. The phylogenetic placement of H. galeatus in the superfamily Tylenchoidea was also examined.

Results

The average haploid genome size estimated using a k-mer approach was 517.69 Mbp. The parti

SUBMITTER: Ma X 

PROVIDER: S-EPMC8781444 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature