Skin tape sampling technique identifies proinflammatory cytokines in atopic dermatitis skin.

ABSTRACT:

Background

Monitoring the effects of biologic therapies in skin diseases will benefit from alternative noninvasive skin sampling techniques to evaluate immune pathways in diseased tissue early and longitudinally.

Objective

To establish a minimally invasive profiling of skin cytokines for diagnosis, therapeutic response monitoring, and clinical research in atopic dermatitis (AD) and other skin diseases, particularly in pediatric cohorts.

Methods

We developed a novel method for cytokine profiling in the epidermis using skin tape strips (STSs) in a setting designed to maximize the efficiency of protein extraction from STSs. This method was applied to analyze STS protein extracts from the lesional skin of children having AD (n = 41) and normal, healthy controls (n = 22).

SUBMITTER: Lyubchenko T 

PROVIDER: S-EPMC8782053 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature