Prokaryotic rRNA-mRNA interactions are involved in all translation steps and shape bacterial transcripts.

ABSTRACT: The well-established Shine-Dalgarno model suggests that translation initiation in bacteria is regulated via base-pairing between ribosomal RNA (rRNA) and mRNA. We used novel computational analyses and modelling of 823 bacterial genomes coupled with experiments to demonstrate that rRNA-mRNA interactions are diverse and regulate all translation steps from pre-initiation to termination. Previous research has reported the significant influence of rRNA-mRNA interactions, mainly in the initiation phase of translation. The results reported in this paper suggest that, in addition to the rRNA-mRNA interactions near the start codon that trigger initiation in bacteria, rRNA-mRNA interactions affect all sub-stages of the translation process (pre-initiation, initiation, elongation, termination). As the

SUBMITTER: Bahiri Elitzur S 

PROVIDER: S-EPMC8782165 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature