MRNA m5C controls adipogenesis by promoting CDKN1A mRNA export and translation.

ABSTRACT: 5-Methylcytosine (m⁵C) is a type of RNA modification that exists in tRNAs and rRNAs and was recently found in mRNA. Although mRNA m⁵C modification has been reported to regulate diverse biological process, its function in adipogenesis remains unknown. Here, we demonstrated that knockdown of NOL1/NOP2/Sun domain family member 2 (NSUN2), a m⁵C methyltransferase, increased lipid accumulation of 3T3-L1 preadipocytes through accelerating cell cycle progression during mitotic clonal expansion (MCE) at the early stage of adipogenesis. Mechanistically, we proved that NSUN2 directly targeted cyclin-dependent kinase inhibitor 1A (CDKN1A) mRNA, a key inhibitory regulator of cell cycle progression, and upregulated its protein expression in an m⁵C-depen

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8782175 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature