BIOME-Preserve: A novel storage and transport medium for preserving anaerobic microbiota samples for culture recovery.

ABSTRACT: Sequencing-based protocols for studying the human microbiome have unearthed a wealth of information about the relationship between the microbiome and human health. But these microbes cannot be leveraged as therapeutic targets without culture-based studies to phenotype species of interest and to establish culture collections for use in animal models. Traditional sample collection protocols are focused on preserving nucleic acids and metabolites and are largely inappropriate for preserving sensitive anaerobic bacteria for later culture recovery. Here we introduce a novel microbiome preservation kit (BIOME-Preserve) that facilitates recovery of anaerobic bacteria from human stool. Using a combination of culture recovery and shallow whole-genome shotgun sequencing, we characterized the anaerob

SUBMITTER: Hyde ER 

PROVIDER: S-EPMC8782539 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature